Human CKMT2 ELISA Kit

Alternative name
Human basic-type mitochondrial creatine kinase ELISA Kit;Human mib-CK ELISA Kit;Human sarcomeric mitochondrial creatine kinase ELISA Kit;Human S-MtCK ELISA Kit;Human SMTCK ELISA Kit;Human creatine kinase, mitochondrial 2 ELISA Kit;Human creatine kinase S-type, mitochondrial ELISA Kit;Human creatine kinase, mitochondrial 2 (sarcomeric) ELISA Kit;
Catalog
E010186
species
Human
GeneCKMT2
SpecificityThis assay has high sensitivity and excellent specificity for detection of CKMT2. No significant cross-reactivity or interference between CKMT2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CKMT2 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman CKMT2 ELISA Kit allows for the in vitro quantitative determination of CKMT2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalIntended Uses: This CKMT2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CKMT2. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||CKMT2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CKMT2 antibody and an CKMT2-HRP conjugate. The assay sample and buffer are incubated together with CKMT2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CKMT2 concentration since CKMT2 from samples and CKMT2-HRP conjugate compete for the anti-CKMT2 antibody binding site. Since the number of sites is limited, as more sites are occupied by CKMT2 from the sample, fewer sites are left to bind CKMT2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CKMT2 concentration in each sample is interpolated from this standard curve.
Human Creatine kinase S-type, mitochondrial Protein information
Uniprot ID KCRS_HUMAN
Uniprot AC P17540; Q6ICS8; Q8N1E1;
UniGene Hs.80691;
GeneID 1160
KEGG hsa:1160;
Human Creatine kinase S-type, mitochondrial Protein SEQUENCE
SEQUENCE 419 AA; 47504 MW; 0CAF000074D99B6F CRC64;
MASIFSKLLT GRNASLLFAT MGTSVLTTGY LLNRQKVCAE VREQPRLFPP
SADYPDLRKH NNCMAECLTP AIYAKLRNKV TPNGYTLDQC IQTGVDNPGH
PFIKTVGMVA GDEESYEVFA DLFDPVIKLR HNGYDPRVMK HTTDLDASKI
TQGQFDEHYV LSSRVRTGRS IRGLSLPPAC TRAERREVEN VAITALEGLK
GDLAGRYYKL SEMTEQDQQR LIDDHFLFDK PVSPLLTCAG MARDWPDARG
IWHNYDKTFL IWINEEDHTR VISMEKGGNM KRVFERFCRG LKEVERLIQE
RGWEFMWNER LGYILTCPSN LGTGLRAGVH VRIPKLSKDP RFSKILENLR
LQKRGTGGVD TAAVADVYDI SNIDRIGRSE VELVQIVIDG VNYLVDCEKK
LERGQDIKVP PPLPQFGKK
UCSC uc003khc.5; human.;

Category

Human CKMT2 ELISA Kit