Human CK-MM ELISA Kit

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  • Catalog
    E010185
  • species
    Human
  • GeneCK-MM
  • Other Species Human CK MM ELISA KitMouse CK-MM ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human CK-MM. No significant cross-reactivity or interference between human CK-MM and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human CK-MM is typically less than 0.78 mU/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the me
  • Intended UseHuman CK-MM ELISA Kit allows for the in vitro quantitative determination of CK-MM , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CK-MM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CK-MM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CK-MM is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CK-MM bound in the initial step. The color development is stopped and the intensity of the color is measured.



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