Human ADAM15 ELISA Kit

Alternative name
Human ADAM 15 ELISA Kit;Human metalloprotease RGD disintegrin protein ELISA Kit;Human metalloproteinase-like, disintegrin-like, and cysteine-rich protein 15 ELISA Kit;Human MDC-15 ELISA Kit;Human Metargidin ELISA Kit;Human MDC15 ELISA Kit;Human ADAM metallopeptidase domain 15 ELISA Kit;Human disintegrin and metalloproteinase domain-containing protein 15 ELISA Kit;Human a disintegrin and metalloproteinase domain 15 (metargidin) ELISA Kit;
Catalog
E000010
species
Human
GeneADAM15
Standard Curve
Other Species Mouse Adam15 ELISA Kit Rat Adam15 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of A Disintegrin And Metalloprotease 15 (ADAM15). No significant cross-reactivity or interference between A Disintegrin And Metalloprotease 15 (ADAM15) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.054ng/mL.
Intended UseHuman ADAM15 ELISA Kit allows for the in vitro quantitative determination of ADAM15 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to A Disintegrin And Metalloprotease 15 (ADAM15). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to A Disintegrin And Metalloprotease 15 (ADAM15). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain A Disintegrin And Metalloprotease 15 (ADAM15), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of A Disintegrin And Metalloprotease 15 (ADAM15) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Disintegrin and metalloproteinase domain-containing protein 15 Protein information
Uniprot ID ADA15_HUMAN
Uniprot AC Q71S67; Q71S68; Q71S69; Q96C78; U3KQL5;
UniGene Hs.312098;
GeneID 8751
KEGG hsa:8751;
Human Disintegrin and metalloproteinase domain-containing protein 15 Protein SEQUENCE
SEQUENCE 863 AA; 92959 MW; 004936E9182629CA CRC64;
MRLALLWALG LLGAGSPLPS WPLPNIGGTE EQQAESEKAP REPLEPQVLQ
DDLPISLKKV LQTSLPEPLR IKLELDGDSH ILELLQNREL VPGRPTLVWY
QPDGTRVVSE GHTLENCCYQ GRVRGYAGSW VSICTCSGLR GLVVLTPERS
YTLEQGPGDL QGPPIISRIQ DLHLPGHTCA LSWRESVHTQ KPPEHPLGQR
HIRRRRDVVT ETKTVELVIV ADHSEAQKYR DFQHLLNRTL EVALLLDTFF
RPLNVRVALV GLEAWTQRDL VEISPNPAVT LENFLHWRRA HLLPRLPHDS
AQLVTGTSFS GPTVGMAIQN SICSPDFSGG VNMDHSTSIL GVASSIAHEL
GHSLGLDHDL PGNSCPCPGP APAKTCIMEA STDFLPGLNF SNCSRRALEK
ALLDGMGSCL FERLPSLPPM AAFCGNMFVE PGEQCDCGFL DDCVDPCCDS
LTCQLRPGAQ CASDGPCCQN CQLRPSGWQC RPTRGDCDLP EFCPGDSSQC
PPDVSLGDGE PCAGGQAVCM HGRCASYAQQ CQSLWGPGAQ PAAPLCLQTA
NTRGNAFGSC GRNPSGSYVS CTPRDAICGQ LQCQTGRTQP LLGSIRDLLW
ETIDVNGTEL NCSWVHLDLG SDVAQPLLTL PGTACGPGLV CIDHRCQRVD
LLGAQECRSK CHGHGVCDSN RHCYCEEGWA PPDCTTQLKA TSSLTTGLLL
SLLVLLVLVM LGASYWYRAR LHQRLCQLKG PTCQYRAAQS GPSERPGPPQ
RALLARGTKQ ASALSFPAPP SRPLPPDPVS KRLQAELADR PNPPTRPLPA
DPVVRSPKSQ GPAKPPPPRK PLPADPQGRC PSGDLPGPGA GIPPLVVPSR
PAPPPPTVSS LYL
UCSC uc001fgr.3; human. [Q13444-1];

Category

Human ADAM15 ELISA Kit