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Catalog
C000997
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Alternative name
Pyruvate Dehydrogenase Alpha CLIA KIT; Pyruvate Dehydrogenase Alpha CLIA KIT; Pyruvate dehydrogenase E1 component subunit alphapdhA CLIA KIT;
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GenePDHa
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Other Species
Mouse PDHa clia kitRat PDHA clia kit
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Species ReactivityHuman
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Detection Range0.156~10ng/mL
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Sensitivity0.094ng/mL
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Product Description specificalThe micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PDHalpha. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PDHalpha and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PDHalpha, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit(RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PDHalpha. You can calculate the concentration of Human PDHalpha in the samples by comparing the RLU value of the samples to the standard curve.
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