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Catalog
C000942
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Alternative name
Phospholipase A2, membrane associated CLIA KIT; GIIC sPLA2 CLIA KIT; Group IIA phospholipase A2 CLIA KIT; Non-pancreatic secretory phospholipase A2 CLIA KIT; NPS-PLA2 CLIA KIT; Phosphatidylcholine 2-acylhydrolase 2APLA2G2A CLIA KIT; PLA2B CLIA KIT; PLA2L CLIA KIT; RASF-A CLIA KIT; NPS-PLA2 CLIA KIT
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GeneLpPLA2
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Other Species
Human LpPLA2 clia kit
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Species ReactivityHuman
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Phospholipase A2, Lipoprotein Associated (LpPLA2).
No significant cross-reactivity or interference between Phospholipase A2, Lipoprotein Associated (LpPLA2) and analogues was observed.
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SamplesSerum, plasma and other biological fluids
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Detection Range13.72-10000pg/mL
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SensitivityThe minimum detectable dose of this kit is typically less than 4.81pg/mL.
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PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Phospholipase A2, Lipoprotein Associated (LpPLA2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Phospholipase A2, Lipoprotein Associated (LpPLA2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
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Product Description specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Phospholipase A2, Lipoprotein Associated (LpPLA2) level in the sample or standard.
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