Mouse PEDF clia kit

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  • Catalog
    C000926
  • Alternative name

    Pigment epithelium-derived factor CLIA KIT; Cell proliferation-inducing gene 35 protein CLIA KIT; EPC-1 CLIA KIT; Serpin F1SERPINF1 CLIA KIT; PEDF CLIA KIT; PEDF CLIA KIT

  • GenePEDF
  • Other Species Rat PEDF clia kitHuman PEDF clia kitMouse PEDF clia kitHuman PEDF clia kit
  • Species ReactivityMouse
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Pigment Epithelium Derived Factor (PEDF). No significant cross-reactivity or interference between Pigment Epithelium Derived Factor (PEDF) and analogues was observed.
  • SamplesSerum, plasma and other biological fluids
  • Detection Range0.14-100ng/mL
  • SensitivityThe minimum detectable dose of this kit is typically less than 0.04ng/mL.
  • PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Pigment Epithelium Derived Factor (PEDF) were tested 20 times on one plate, respectively.
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pigment Epithelium Derived Factor (PEDF) were tested on 3 different plates, 8 replicates in each plate.
    CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
  • Product Description specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Pigment Epithelium Derived Factor (PEDF) level in the sample or standard.


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