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Catalog
C000872
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Alternative name
Platelet-derived growth factor D CLIA KIT; Iris-expressed growth factor CLIA KIT; Spinal cord-derived growth factor BPDGFD CLIA KIT; IEGF CLIA KIT; SCDGFB CLIA KIT; PDGF-D CLIA KIT; SCDGF-B CLIA KIT; PDGFD latent form CLIA KIT
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GenePDGFD
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Other Species
Rat PDGFD clia kitHuman PDGFD clia kitMouse PDGFD clia kit
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Platelet Derived Growth Factor D (PDGFD)
No significant cross-reactivity or interference between Platelet Derived Growth Factor D (PDGFD) and analogues was observed.
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SamplesPlasma
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Detection Range0.027-20ng/mL The standard curve concentrations used for the ELISA's were 20ng/mL, 6.67ng/mL, 2.22ng/mL, 0.74ng/mL, 0.247ng/mL, 0.082ng/mL, 0.027ng/mL
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SensitivityThe minimum detectable dose of this kit is typically less than 0.117ng/mL
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PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Platelet Derived Growth Factor D (PDGFD) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Platelet Derived Growth Factor D (PDGFD) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
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Product Description specificalThe microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Platelet Derived Growth Factor D (PDGFD) level in the sample or standard.
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