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Catalog
C000838
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GenePARP
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Species ReactivityHuman
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Poly ADP Ribose Polymerase (PARP).
No significant cross-reactivity or interference between Poly ADP Ribose Polymerase (PARP) and analogues was observed.
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SamplesTissue homogenates, cell lysates and other biological fluids
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Detection Range0.27-200ng/mL
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SensitivityThe minimum detectable dose of this kit is typically less than 0.08ng/mL.
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PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Poly ADP Ribose Polymerase (PARP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Poly ADP Ribose Polymerase (PARP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
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Product Description specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Poly ADP Ribose Polymerase (PARP) level in the sample or standard.
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