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Catalog
C000792
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Alternative name
Vitamin K-dependent protein C CLIA KIT; Anticoagulant protein C CLIA KIT; Autoprothrombin IIA CLIA KIT; Blood coagulation factor XIVPROC CLIA KIT;
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GenePROC
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Other Species
Rat PROC clia kit
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Species ReactivityHuman
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SpecificityThis kit recognizes natural and recombinant PROC. No significant cross-reactivity or interference between PROC and analogues was observed.
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SamplesSerum, plasma and other biological fluids.
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Detection Range15.625-1000pg/mL
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Sensitivity9.375pg/mL
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Product Description specificalIntended Uses: This CLIA kit applies to the in vitro quantitative determination of PROC concentrations in serum, plasma and other biological fluids.
Principle of the Assay||This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to PROC. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PROC and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PROC, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of PROC. You can calculate the concentration of PROC in the samples by comparing the RLU value of the samples to the standard curve.
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