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Catalog
C000784
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GenePG
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Other Species
Rat Pg clia kitHuman Pg clia kitMouse Pg clia kit
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Species ReactivityAll
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Progesterone (PG).
No significant cross-reactivity or interference between Progesterone (PG) and analogues was observed.
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SamplesSerum, plasma and other biological fluids
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Detection Range1.17-300ng/mL
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SensitivityThe minimum detectable dose of this kit is typically less than 0.43ng/mL.
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PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Progesterone (PG) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Progesterone (PG) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
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Product Description specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with a monoclonal antigen. A competitive inhibition reaction is launched between biotin labeled Progesterone (PG) and unlabeled Progesterone (PG) (Standards or samples) with the pre-coated secondary antibody. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Progesterone (PG) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Progesterone (PG) level in the sample or standard.
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