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Catalog
C000717
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Alternative name
Ras-related protein Rab-5A CLIA KIT; RAB5A CLIA KIT; RAB5 CLIA KIT
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GeneRAB5A
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Other Species
Human RAB5A clia kit
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Species ReactivityMouse
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SpecificityThis assay has high sensitivity and excellent specificity for detection of RAB5A, Member RAS Oncogene Family (RAB5A).
No significant cross-reactivity or interference between RAB5A, Member RAS Oncogene Family (RAB5A) and analogues was observed.
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SamplesTissue homogenates and other biological fluids
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Detection Range13.72-10000pg/mL
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SensitivityThe minimum detectable dose of this kit is typically less than 6.37pg/mL.
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PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level RAB5A, Member RAS Oncogene Family (RAB5A) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level RAB5A, Member RAS Oncogene Family (RAB5A) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
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Product Description specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the RAB5A, Member RAS Oncogene Family (RAB5A) level in the sample or standard.
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