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Catalog
C000676
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Alternative name
Rho guanine nucleotide exchange factor 25 CLIA KIT; Guanine nucleotide exchange factor GEFT CLIA KIT; Rac/Cdc42/Rho exchange factor GEFT CLIA KIT; RhoA/Rac/Cdc42 guanine nucleotide exchange factor GEFT CLIA KIT; p63RhoGEFARHGEF25 CLIA KIT; GEFT CLIA KIT
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GeneARHGEF25
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Other Species
ARHGEF25 clia kit ARHGEF25 clia kit
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Rho Guanine Nucleotide Exchange Factor 25 (ARHGEF25)
No significant cross-reactivity or interference between Rho Guanine Nucleotide Exchange Factor 25 (ARHGEF25) and analogues was observed.
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SamplesTissue homogenates and other biological fluids
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Detection Range0.014-10ng/mL The standard curve concentrations used for the ELISA's were 10ng/mL, 3.33ng/mL, 1.11ng/mL, 0.37ng/mL, 0.123ng/mL, 0.041ng/mL, 0.014ng/mL
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SensitivityThe minimum detectable dose of this kit is typically less than 0.062ng/mL
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PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Rho Guanine Nucleotide Exchange Factor 25 (ARHGEF25) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Rho Guanine Nucleotide Exchange Factor 25 (ARHGEF25) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
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Product Description specificalThe microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Rho Guanine Nucleotide Exchange Factor 25 (ARHGEF25) level in the sample or standard.
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