Protein S100-B CLIA KIT; S-100 protein beta chain CLIA KIT; S-100 protein subunit beta CLIA KIT; S100 calcium-binding protein BS100B CLIA KIT;
Catalog
C000635
species
Human
GeneS100B
SpecificityThis kit recognizes natural and recombinant S100B. No significant cross-reactivity or interference between S100B and analogues was observed.
SamplesSerum, plasma and other biological fluids.
Sensitivity9.375pg/mL
Intended UseHuman S100B clia kit allows for the in vitro quantitative determination of S100B , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalIntended Uses: This CLIA kit applies to the in vitro quantitative determination of S100B concentrations in serum, plasma and other biological fluids.
Principle of the Assay||This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to S100B. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for S100B and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain S100B, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of S100B. You can calculate the concentration of S100B in the samples by comparing the RLU value of the samples to the standard curve.
No manual or datasheet available for this product.