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Catalog
C000635
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Alternative name
Protein S100-B CLIA KIT; S-100 protein beta chain CLIA KIT; S-100 protein subunit beta CLIA KIT; S100 calcium-binding protein BS100B CLIA KIT;
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GeneS100B
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Other Species
Mouse S100B clia kitRat S100B clia kitHuman S100B clia kit
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Species ReactivityHuman
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SpecificityThis kit recognizes natural and recombinant S100B. No significant cross-reactivity or interference between S100B and analogues was observed.
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SamplesSerum, plasma and other biological fluids.
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Detection Range15.625-1000pg/mL
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Sensitivity9.375pg/mL
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Product Description specificalIntended Uses: This CLIA kit applies to the in vitro quantitative determination of S100B concentrations in serum, plasma and other biological fluids.
Principle of the Assay||This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to S100B. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for S100B and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain S100B, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of S100B. You can calculate the concentration of S100B in the samples by comparing the RLU value of the samples to the standard curve.
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