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Catalog
C000568
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Alternative name
STE20/SPS1-related proline-alanine-rich protein kinase CLIA KIT; DCHT CLIA KIT; Serine/threonine-protein kinase 39STK39 CLIA KIT; SPAK CLIA KIT; Ste-20-related kinase CLIA KIT
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GeneSTK39
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Other Species
Human STK39 clia kit
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Species ReactivityHuman
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Serine/Threonine Kinase 39 (STK39).
No significant cross-reactivity or interference between Serine/Threonine Kinase 39 (STK39) and analogues was observed.
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SamplesTissue homogenates and other biological fluids
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Detection Range0.069-50ng/mL
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SensitivityThe minimum detectable dose of this kit is typically less than 0.03ng/mL.
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PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Serine/Threonine Kinase 39 (STK39) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Serine/Threonine Kinase 39 (STK39) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
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Product Description specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Serine/Threonine Kinase 39 (STK39) level in the sample or standard.
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