Sex hormone binding globulin CLIA KIT; Sex hormone binding globulinSHBG CLIA KIT;
Catalog
C000556
species
Human
GeneSHBG
SpecificityThis kit recognizes natural and recombinant SHBG. No significant cross-reactivity or interference between SHBG and analogues was observed.
SamplesSerum, plasma and other biological fluids
Sensitivity0.75nmol/L
Intended UseHuman SHBG clia kit allows for the in vitro quantitative determination of SHBG , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalIntended Uses: This CLIA kit applies to the in vitro quantitative determination of SHBG concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to SHBG. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for SHBG and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain SHBG, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of SHBG. You can calculate the concentration of SHBG in the samples by comparing the RLU value of the samples to the standard curve.
No manual or datasheet available for this product.