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Catalog
C000053
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Alternative name
Protein Wnt-10b CLIA KIT; Protein Wnt-12WNT10B CLIA KIT; WNT12 CLIA KIT
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GeneWNT10B
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Other Species
Human WNT10B clia kit
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Species ReactivityMouse
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Wingless Type MMTV Integration Site Family, Member 10B (WNT10B).
No significant cross-reactivity or interference between Wingless Type MMTV Integration Site Family, Member 10B (WNT10B) and analogues was observed.
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SamplesSerum, plasma, tissue homogenates and other biological fluids
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Detection Range13.72-10000pg/mL
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SensitivityThe minimum detectable dose of this kit is typically less than 6.31pg/mL.
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PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wingless Type MMTV Integration Site Family, Member 10B (WNT10B) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wingless Type MMTV Integration Site Family, Member 10B (WNT10B) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
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Product Description specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Wingless Type MMTV Integration Site Family, Member 10B (WNT10B) level in the sample or standard.
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