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Catalog
C000508
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Alternative name
Substance P CLIA KIT; Substance P
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GeneSP
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Other Species
Rat SP clia kitGuinea Pig SP clia kitMouse SP clia kitHuman SP clia kitBovine SP clia kitCanine SP clia kitMouse SP-D clia kitMouse SP-A clia kit
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Species ReactivityHuman
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SpecificityThis kit recognizes natural and recombinant Human SP. No significant cross-reactivity or interference between Human SP and analogues was observed.
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SamplesSerum, plasma and other biological fluids
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Detection Range15.625-1000pg/mL
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Sensitivity9.375pg/mL
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Product Description specificalIntended Uses: This CLIA kit applies to the in vitro quantitative determination of Human SP concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This CLIA kit uses Competitive-CLIA as the method. The microtiter plate provided in this kit has been pre-coated with Human SP. During the reaction, Human SP in the sample or standard competes with a fixed amount of Human SP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human SP. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The Relative light unit (RLU) valueis measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is negatively associated with the concentration of Human SP. You can calculate the concentration of Human SP in the samples by comparing the RLU of the samples to the standard curve.
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