Pulmonary surfactant-associated protein B CLIA KIT; 6 kDa protein CLIA KIT; Pulmonary surfactant-associated proteolipid SPL(Phe)SFTPB CLIA KIT; SFTP3 CLIA KIT; SP-B CLIA KIT
Product Description specificalThe micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human SPB. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SPB and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SPB, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit(RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human SPB. You can calculate the concentration of Human SPB in the samples by comparing the RLU value of the samples to the standard curve.