SpecificityThis assay has high sensitivity and excellent specificity for detection of WNT3A. 100% cross-reactivity of WNT3A was observed among human, mouse and rat.
SensitivityThe minimum detectable dose of WNT3A is typically less than 20.5pg/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean RLU value of 20 replicates of the zero standard added by their two standard deviations.
Intended UseRat WNT3A clia kit allows for the in vitro quantitative determination of WNT3A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to WNT3A. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to WNT3A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the WNT3A level in the sample or standard.
No manual or datasheet available for this product.