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Catalog
C000494
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Alternative name
Speckle-type POZ protein CLIA KIT; HIB homolog 1 CLIA KIT; Roadkill homolog 1SPOP CLIA KIT;
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GeneSPOP
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Other Species
SPOP clia kit SPOP clia kit
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Speckle Type POZ Protein (SPOP)
No significant cross-reactivity or interference between Speckle Type POZ Protein (SPOP) and analogues was observed.
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SamplesTissue homogenates and other biological fluids
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Detection Range6.859-5000pg/mL The standard curve concentrations used for the ELISA's were 5000pg/mL, 1666.67pg/mL, 555.56pg/mL, 185.19pg/mL, 61.728pg/mL, 20.576pg/mL, 6.859pg/mL
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SensitivityThe minimum detectable dose of this kit is typically less than 29pg/mL
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PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Speckle Type POZ Protein (SPOP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Speckle Type POZ Protein (SPOP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
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Product Description specificalThe microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Speckle Type POZ Protein (SPOP) level in the sample or standard.
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