SpecificityThis assay has high sensitivity and excellent specificity for detection of Wingless Type MMTV Integration Site Family, Member 4 (WNT4).
No significant cross-reactivity or interference between Wingless Type MMTV Integration Site Family, Member 4 (WNT4) and analogues was observed.
SamplesTissue homogenates and other biological fluids
SensitivityThe minimum detectable dose of this kit is typically less than 5.29pg/mL.
Intended UseRat WNT4 clia kit allows for the in vitro quantitative determination of WNT4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Wingless Type MMTV Integration Site Family, Member 4 (WNT4) level in the sample or standard.
No manual or datasheet available for this product.