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Catalog
C000461
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Alternative name
Extracellular superoxide dismutase [Cu-Zn] CLIA KIT; SOD3 CLIA KIT; EC-SOD CLIA KIT
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GeneSOD3
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Other Species
Rat SOD3 clia kit
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Species ReactivityMouse
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Superoxide Dismutase 3, Extracellular (SOD3).
No significant cross-reactivity or interference between Superoxide Dismutase 3, Extracellular (SOD3) and analogues was observed.
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SamplesSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
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Detection Range41.15-30000pg/mL
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SensitivityThe minimum detectable dose of this kit is typically less than 18.01pg/mL.
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PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Superoxide Dismutase 3, Extracellular (SOD3) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Superoxide Dismutase 3, Extracellular (SOD3) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
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Product Description specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Superoxide Dismutase 3, Extracellular (SOD3) level in the sample or standard.
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