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Catalog
C000043
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Alternative name
Protein Wnt-5b CLIA KIT; WNT5B CLIA KIT;
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GeneWNT5B
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Species ReactivityHuman
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Wingless Type MMTV Integration Site Family, Member 5B (WNT5B).
No significant cross-reactivity or interference between Wingless Type MMTV Integration Site Family, Member 5B (WNT5B) and analogues was observed.
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SamplesSerum, plasma, tissue homogenates and other biological fluids
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Detection Range0.14-100ng/mL
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SensitivityThe minimum detectable dose of this kit is typically less than 0.07ng/mL.
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PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wingless Type MMTV Integration Site Family, Member 5B (WNT5B) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wingless Type MMTV Integration Site Family, Member 5B (WNT5B) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
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Product Description specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Wingless Type MMTV Integration Site Family, Member 5B (WNT5B) level in the sample or standard.
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