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Catalog
C000404
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GeneTesto
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Species ReactivityAll
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Testo. No significant cross-reactivity or interference between Testo and analogues was observed.
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Detection Range78.1-20,000pg/mL. The standard curve concentrations used for the CLIA's were 20,000pg/mL, 5,000pg/mL, 1,250pg/mL, 312.5pg/mL, 78.1pg/mL.
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SensitivityThe minimum detectable dose of Testo is typically less than 27.7pg/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean RLU value of 20 replicates of the zero standard subtracted by their two standard deviations.
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Product Description specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to Testo. A competitive inhibition reaction is launched between biotin labeled Testo and unlabeled Testo (Standards or samples) with the pre-coated antibody specific to Testo. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Testo in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Testo level in the sample or standard.
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