SpecificityThis assay has high sensitivity and excellent specificity for detection of Adiponectin (ADP).
No significant cross-reactivity or interference between Adiponectin (ADP) and analogues was observed.
SamplesSerum, plasma, tissue homogenates, cell culture supernates and other biological fluids
SensitivityThe minimum detectable dose of this kit is typically less than 5.15pg/mL.
PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adiponectin (ADP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adiponectin (ADP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Product Description specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Adiponectin (ADP) level in the sample or standard.