Human ACTH clia kit

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  • Alternative name

    Pro-opiomelanocortin CLIA KIT; Corticotropin-lipotropinPomc CLIA KIT; Pomc1 CLIA KIT; POMC CLIA KIT; ACTH CLIA KIT; CLIP CLIA KIT

  • GeneACTH
  •  Human ACTH clia kit
  • Other Species Mouse ACTH clia kitRat ACTH clia kitHuman ACTH clia kitMouse ACTH clia kitRat ACTH clia kit
  • Species ReactivityHuman
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Adrenocorticotropic Hormone (ACTH). No significant cross-reactivity or interference between Adrenocorticotropic Hormone (ACTH) and analogues was observed.
  • SamplesSerum, plasma, tissue homogenates, cell culture supernates and other biological fluids
  • Detection Range3.91-1000pg/mL
  • SensitivityThe minimum detectable dose of this kit is typically less than 1.82pg/mL.
  • PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adrenocorticotropic Hormone (ACTH) were tested 20 times on one plate, respectively.
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adrenocorticotropic Hormone (ACTH) were tested on 3 different plates, 8 replicates in each plate.
    CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
  • Product Description specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with a monoclonal antigen. A competitive inhibition reaction is launched between biotin labeled Adrenocorticotropic Hormone (ACTH) and unlabeled Adrenocorticotropic Hormone (ACTH) (Standards or samples) with the pre-coated secondary antibody. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Adrenocorticotropic Hormone (ACTH) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Adrenocorticotropic Hormone (ACTH) level in the sample or standard.

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