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Catalog
C003894
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Alternative name
Autoimmune regulator CLIA KIT; Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein CLIA KIT; APECED proteinAIRE CLIA KIT; APECED CLIA KIT; APECED protein CLIA KIT
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GeneAIRE
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Species ReactivityHuman
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Detection Range0.156~10ng/mL
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Sensitivity0.094ng/mL
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Product Description specificalThe micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human AIRE. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AIRE and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AIRE, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit(RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human AIRE. You can calculate the concentration of Human AIRE in the samples by comparing the RLU value of the samples to the standard curve.
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