Bovine ALP clia kit

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  • Catalog
    C003880
  • Alternative name

    Alkaline phosphatase, tissue-nonspecific isozyme CLIA KIT; Alkaline phosphatase liver/bone/kidney isozymeALPL CLIA KIT; AP-TNAP CLIA KIT; TNSALP CLIA KIT

  • GeneALP
  •  Bovine ALP clia kit
  • Other Species Mouse ALP clia kitRat ALP clia kitHuman ALP clia kit
  • Species ReactivityBovine
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Alkaline Phosphatase (ALP). No significant cross-reactivity or interference between Alkaline Phosphatase (ALP) and analogues was observed.
  • SamplesSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Detection Range41.15-30000pg/mL
  • PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alkaline Phosphatase (ALP) were tested 20 times on one plate, respectively.
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alkaline Phosphatase (ALP) were tested on 3 different plates, 8 replicates in each plate.
    CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
  • Product Description specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Alkaline Phosphatase (ALP) level in the sample or standard.



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