SpecificityThis assay has high sensitivity and excellent specificity for detection of Angiotensin I (AngI).
No significant cross-reactivity or interference between Angiotensin I (AngI) and analogues was observed.
SamplesSerum, plasma, tissue homogenates, cell culture supernates and other biological fluids
SensitivityThe minimum detectable dose of this kit is typically less than 2.92pg/mL.
Intended UseHuman AngI clia kit allows for the in vitro quantitative determination of AngI , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with a monoclonal antigen. A competitive inhibition reaction is launched between biotin labeled Angiotensin I (AngI) and unlabeled Angiotensin I (AngI) (Standards or samples) with the pre-coated secondary antibody. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Angiotensin I (AngI) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Angiotensin I (AngI) level in the sample or standard.
No manual or datasheet available for this product.