SpecificityThis assay has high sensitivity and excellent specificity for detection of Bcl2. No significant cross-reactivity or interference between Bcl2 and analogues was observed.
SensitivityThe minimum detectable dose of Bcl2 is typically less than 0.17ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean RLU value of 20 replicates of the zero standard added by their two standard deviations.
Intended UseHuman Bcl2 clia kit allows for the in vitro quantitative determination of Bcl2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bcl2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bcl2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Bcl2 level in the sample or standard.
No manual or datasheet available for this product.