Rat BNIP3 clia kit

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  • Catalog
    C003621
  • Alternative name

    BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 CLIA KIT; BNIP3 CLIA KIT; NIP3 CLIA KIT

  • GeneBNIP3
  • Species ReactivityRat
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Bcl2/Adenovirus E1B 19kDa Interacting Protein 3 (BNIP3). No significant cross-reactivity or interference between Bcl2/Adenovirus E1B 19kDa Interacting Protein 3 (BNIP3) and analogues was observed.
  • SamplesTissue homogenates, cell lysates and other biological fluids
  • Detection Range0.055-40ng/mL
  • SensitivityThe minimum detectable dose of this kit is typically less than 0.03ng/mL.
  • PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bcl2/Adenovirus E1B 19kDa Interacting Protein 3 (BNIP3) were tested 20 times on one plate, respectively.
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bcl2/Adenovirus E1B 19kDa Interacting Protein 3 (BNIP3) were tested on 3 different plates, 8 replicates in each plate.
    CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
  • Product Description specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Bcl2/Adenovirus E1B 19kDa Interacting Protein 3 (BNIP3) level in the sample or standard.


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