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Catalog
C000346
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Alternative name
Thyrotroph embryonic factor CLIA KIT; TEF CLIA KIT; KIAA1655 CLIA KIT
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GeneTEF
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Other Species
TEF clia kit TEF clia kit
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Thyrotroph Embryonic Factor (TEF)
No significant cross-reactivity or interference between Thyrotroph Embryonic Factor (TEF) and analogues was observed.
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SamplesTissue homogenates and other biological fluids
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Detection Range6.859-5000pg/mL The standard curve concentrations used for the ELISA's were 5000pg/mL, 1666.67pg/mL, 555.56pg/mL, 185.19pg/mL, 61.728pg/mL, 20.576pg/mL, 6.859pg/mL
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SensitivityThe minimum detectable dose of this kit is typically less than 37pg/mL
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PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thyrotroph Embryonic Factor (TEF) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thyrotroph Embryonic Factor (TEF) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
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Product Description specificalThe microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Thyrotroph Embryonic Factor (TEF) level in the sample or standard.
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