Intended UseRat DHEA clia kit allows for the in vitro quantitative determination of DHEA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalThis CLIA kit uses Competitive-CLiA as the method. The microtiter plate provided in this kit has been pre-coated with Rat DHEA. During the reaction, Rat DHEA in the sample or standard competes with a fixed amount of Rat DHEA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat DHEA. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The Relative light unit(RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is negatively associated with the concentration of Rat DHEA. You can calculate the concentration of Rat DHEA in the samples by comparing the RLU of the samples to the standard curve.
No manual or datasheet available for this product.