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Catalog
C002886
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Alternative name
Pro-epidermal growth factor CLIA KIT; Epidermal growth factorAlternative name(s):UrogastroneEGF CLIA KIT; EGF CLIA KIT
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GeneEGF
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Species ReactivityRat
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Detection Range15.625~1000pg/mL
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Sensitivity9.375pg/mL
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Product Description specificalThe micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat EGF. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat EGF and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat EGF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit(RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat EGF. You can calculate the concentration of Rat EGF in the samples by comparing the RLU value of the samples to the standard curve.
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