SpecificityThis assay has high sensitivity and excellent specificity for detection of EMILIN2. No significant cross-reactivity or interference between EMILIN2 and analogues was observed.
Detection Range13.7-10,000pg/mL. The standard curve concentrations used for the CLIA's were 10,000pg/mL, 3,333.3pg/mL, 1,111.1pg/mL, 370.4pg/mL, 123.5pg/mL, 41.2pg/mL, 13.7pg/mL.
SensitivityThe minimum detectable dose of EMILIN2 is typically less than 4.8pg/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean RLU value of 20 replicates of the zero standard added by their two standard deviations.
Product Description specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to EMILIN2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to EMILIN2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the EMILIN2 level in the sample or standard.