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Catalog
C000278
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Alternative name
Lymphotoxin-alpha CLIA KIT; TNF-beta CLIA KIT; Tumor necrosis factor ligand superfamily member 1LTA CLIA KIT; TNFB CLIA KIT; TNFSF1 CLIA KIT; LT-alpha CLIA KIT
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GeneTNF-b
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Other Species
Human TNF-b clia kitMouse TNF-b clia kit
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Species ReactivityRat
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Detection Range31.25~2000pg/mL
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Sensitivity18.75pg/mL
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Product Description specificalThe micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat TNF-beta. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TNF-beta and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TNF-beta, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit(RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat TNF-beta. You can calculate the concentration of Rat TNF-beta in the samples by comparing the RLU value of the samples to the standard curve.
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