SpecificityThis assay has high sensitivity and excellent specificity for detection of E1. No significant cross-reactivity or interference between E1 and analogues was observed.
Detection Range23.44-6,000pg/mL. The standard curve concentrations used for the CLIA's were 6,000pg/mL, 1,500pg/mL, 375pg/mL, 93.75pg/mL, 23.44pg/mL.
SensitivityThe minimum detectable dose of E1 is typically less than 7.96pg/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean RLU value of 20 replicates of the zero standard subtracted by their two standard deviations.
Product Description specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to E1. A competitive inhibition reaction is launched between biotin labeled E1 and unlabeled E1 (Standards or samples) with the pre-coated antibody specific to E1. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of E1 in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the E1 level in the sample or standard.