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Catalog
C000266
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Alternative name
Troponin I, slow skeletal muscle CLIA KIT; Troponin I, slow-twitch isoformTNNI1 CLIA KIT;
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GeneTNNI1
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Species ReactivityHuman
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SpecificityThis kit recognizes natural and recombinant TNNI1. No significant cross-reactivity or interference between TNNI1 and analogues was observed.
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SamplesSerum, plasma and other biological fluids
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Detection Range12.5-800pg/mL
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Sensitivity7.5pg/mL
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Product Description specificalIntended Uses: This CLIA kit applies to the in vitro quantitative determination of TNNI1 concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to TNNI1. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for TNNI1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TNNI1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of TNNI1. You can calculate the concentration of TNNI1 in the samples by comparing the RLU value of the samples to the standard curve.
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