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Catalog
C002455
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Alternative name
Growth differentiation factor 5 CLIA KIT; Growth differentiation factor 5Gdf5 CLIA KIT;
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GeneGDF5
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Species ReactivityRat
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Detection Range0.156~10ng/mL
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Sensitivity0.094ng/mL
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Product Description specificalThe micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat GDF5. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GDF5 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GDF5, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit(RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat GDF5. You can calculate the concentration of Rat GDF5 in the samples by comparing the RLU value of the samples to the standard curve.
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