Insulin growth factor-like family member 1 CLIA KIT; IGFL1 CLIA KIT;
SpecificityThis assay has high sensitivity and excellent specificity for detection of IGF Like Family, Member 1 (IGFL1)
No significant cross-reactivity or interference between IGF Like Family, Member 1 (IGFL1) and analogues was observed.
SamplesTissue homogenates and other biological fluids
Detection Range2.743-2000pg/mL The standard curve concentrations used for the ELISA's were 2000pg/mL, 666.67pg/mL, 222.22pg/mL, 74.07pg/mL, 24.691pg/mL, 8.23pg/mL, 2.743pg/mL
SensitivityThe minimum detectable dose of this kit is typically less than 9.8pg/mL
PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IGF Like Family, Member 1 (IGFL1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IGF Like Family, Member 1 (IGFL1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Product Description specificalThe microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the IGF Like Family, Member 1 (IGFL1) level in the sample or standard.