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Catalog
C000195
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Alternative name
Serotransferrin CLIA KIT; Beta-1 metal-binding globulin CLIA KIT; SiderophilinTF CLIA KIT; Transferrin CLIA KIT
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GeneTRF
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Other Species
Rat TRF clia kitMouse TRF clia kitRat TRF clia kitCanine TRF clia kitHuman TRF clia kit
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Species ReactivityHuman
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SpecificityThis kit recognizes natural and recombinant TRF. No significant cross-reactivity or interference between TRF and analogues was observed.
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SamplesSerum, plasma and other biological fluids.
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Detection Range0.313-20ng/mL
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Sensitivity0.188ng/mL
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Product Description specificalIntended Uses: This CLIA kit applies to the in vitro quantitative determination of TRF concentrations in serum, plasma and other biological fluids.
Principle of the Assay||This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to TRF. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for TRF and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TRF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of TRF. You can calculate the concentration of TRF in the samples by comparing the RLU value of the samples to the standard curve.
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