SpecificityThis assay has high sensitivity and excellent specificity for detection of Interferon Alpha 7 (IFNa7)
No significant cross-reactivity or interference between Interferon Alpha 7 (IFNa7) and analogues was observed.
SamplesSerum, plasma and other biological fluids
Detection Range1.372-1000pg/mL The standard curve concentrations used for the ELISA's were 1000pg/mL, 333.33pg/mL, 111.11pg/mL, 37.04pg/mL, 12.346pg/mL, 4.115pg/mL, 1.372pg/mL
SensitivityThe minimum detectable dose of this kit is typically less than 5.5pg/mL
PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interferon Alpha 7 (IFNa7) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interferon Alpha 7 (IFNa7) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Product Description specificalThe microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Interferon Alpha 7 (IFNa7) level in the sample or standard.