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Catalog
C001755
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Alternative name
Interleukin-1 receptor accessory protein CLIA KIT; Interleukin-1 receptor 3IL1RAP CLIA KIT; C3orf13 CLIA KIT; IL1R3 CLIA KIT; IL-1 receptor accessory protein CLIA KIT; IL-1RAcP CLIA KIT; IL-1R-3 CLIA KIT; IL-1R3 CLIA KIT
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GeneIL1RAP
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Interleukin 1 Receptor Accessory Protein (IL1RAP)
No significant cross-reactivity or interference between Interleukin 1 Receptor Accessory Protein (IL1RAP) and analogues was observed.
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SamplesSerum, plasma and other biological fluids
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Detection Range0.027-20ng/mL The standard curve concentrations used for the ELISA's were 20ng/mL, 6.67ng/mL, 2.22ng/mL, 0.74ng/mL, 0.247ng/mL, 0.082ng/mL, 0.027ng/mL
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SensitivityThe minimum detectable dose of this kit is typically less than 0.113ng/mL
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PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 1 Receptor Accessory Protein (IL1RAP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 1 Receptor Accessory Protein (IL1RAP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
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Product Description specificalThe microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Interleukin 1 Receptor Accessory Protein (IL1RAP) level in the sample or standard.
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