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Catalog
C001742
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Alternative name
Interleukin-12 subunit alpha CLIA KIT; Cytotoxic lymphocyte maturation factor 35 kDa subunit CLIA KIT; CLMF p35 CLIA KIT; IL-12 subunit p35 CLIA KIT; NK cell stimulatory factor chain 1 CLIA KIT; NKSF1IL12A CLIA KIT; NKSF1 CLIA KIT; IL-12A CLIA KIT; CLMF p35 CLIA KIT; NKSF1 CLIA KIT
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GeneIL12
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Species ReactivityHuman
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SpecificityThis assay has high sensitivity and excellent specificity for detection of IL12. No significant cross-reactivity or interference between IL12 and analogues was observed.
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Detection Range0.69-500pg/mL. The standard curve concentrations used for the CLIA's were 500pg/mL, 166.67pg/mL, 55.56pg/mL, 18.52pg/mL, 6.17pg/mL, 2.06pg/mL, 0.69pg/mL.
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SensitivityThe minimum detectable dose of IL12 is typically less than 0.28pg/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean RLU value of 20 replicates of the zero standard added by their two standard deviations.
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Product Description specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL12A. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to IL12B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the IL12 level in the sample or standard.
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