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Catalog
C001721
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Alternative name
Interleukin-2 CLIA KIT; T-cell growth factor CLIA KIT; TCGF CLIA KIT; INN: AldesleukinIL2 CLIA KIT; IL-2 CLIA KIT; TCGF CLIA KIT
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GeneIL2
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Other Species
Mouse IL2 clia kitHuman IL2 clia kit
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Species ReactivityRat
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SpecificityThis assay has high sensitivity and excellent specificity for detection of IL2. No significant cross-reactivity or interference between IL2 and analogues was observed.
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Detection Range1.37-1,000pg/mL. The standard curve concentrations used for the CLIA's were 1,000pg/mL, 333.33pg/mL, 111.11pg/mL, 37.04pg/mL, 12.35pg/mL, 4.12pg/mL, 1.37pg/mL.
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SensitivityThe minimum detectable dose of IL2 is typically less than 0.57pg/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean RLU value of 20 replicates of the zero standard added by their two standard deviations.
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Product Description specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to IL2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the IL2 level in the sample or standard.
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