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Catalog
C001705
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GeneIL27w
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Other Species
IL27w clia kit IL27w clia kit
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Interleukin 27 Working Designation (IL27w)
No significant cross-reactivity or interference between Interleukin 27 Working Designation (IL27w) and analogues was observed.
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SamplesTissue homogenates and other biological fluids
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Detection Range1.372-1000pg/mL The standard curve concentrations used for the ELISA's were 1000pg/mL, 333.33pg/mL, 111.11pg/mL, 37.04pg/mL, 12.346pg/mL, 4.115pg/mL, 1.372pg/mL
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SensitivityThe minimum detectable dose of this kit is typically less than 6.5pg/mL
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PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 27 Working Designation (IL27w) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 27 Working Designation (IL27w) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
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Product Description specificalThe microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Interleukin 27 Working Designation (IL27w) level in the sample or standard.
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