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Catalog
C001673
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Alternative name
Interleukin-8 CLIA KIT; C-X-C motif chemokine 8 CLIA KIT; Chemokine (C-X-C motif) ligand 8 CLIA KIT; Emoctakin CLIA KIT; Granulocyte chemotactic protein 1 CLIA KIT; GCP-1CXCL8 CLIA KIT; IL8 CLIA KIT; IL-8 CLIA KIT; GCP-1 CLIA KIT; MDNCF CLIA KIT; MONAP CLIA KIT; NAP-1 CLIA KIT; LYNAP CLIA KIT; NAF CLIA KIT
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GeneIL8
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Other Species
Porcine IL8 clia kitCanine IL8 clia kit
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Species ReactivityHuman
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SpecificityThis assay has high sensitivity and excellent specificity for detection of IL8. No significant cross-reactivity or interference between IL8 and analogues was observed.
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Detection Range1.37-1,000pg/mL. The standard curve concentrations used for the CLIA's were 1,000pg/mL, 333.33pg/mL, 111.11pg/mL, 37.04pg/mL, 12.35pg/mL, 4.12pg/mL, 1.37pg/mL.
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SensitivityThe minimum detectable dose of IL8 is typically less than 0.57pg/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean RLU value of 20 replicates of the zero standard added by their two standard deviations.
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Product Description specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL8. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to IL8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the IL8 level in the sample or standard.
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