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Catalog
C001610
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Alternative name
PC4 and SFRS1-interacting protein CLIA KIT; CLL-associated antigen KW-7 CLIA KIT; Dense fine speckles 70 kDa protein CLIA KIT; DFS 70 CLIA KIT; Lens epithelium-derived growth factor CLIA KIT; Transcriptional coactivator p75/p52PSIP1 CLIA KIT; DFS70 CLIA KIT; LEDGF CLIA KIT; PSIP2 CLIA KIT; DFS 70 CLIA KIT
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GeneLEDGF
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Other Species
LEDGF clia kit LEDGF clia kit
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Lens Epithelium Derived Growth Factor (LEDGF)
No significant cross-reactivity or interference between Lens Epithelium Derived Growth Factor (LEDGF) and analogues was observed.
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SamplesTissue homogenates and other biological fluids
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Detection Range0.014-10ng/mL The standard curve concentrations used for the ELISA's were 10ng/mL, 3.33ng/mL, 1.11ng/mL, 0.37ng/mL, 0.123ng/mL, 0.041ng/mL, 0.014ng/mL
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SensitivityThe minimum detectable dose of this kit is typically less than 0.071ng/mL
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PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lens Epithelium Derived Growth Factor (LEDGF) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lens Epithelium Derived Growth Factor (LEDGF) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
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Product Description specificalThe microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Lens Epithelium Derived Growth Factor (LEDGF) level in the sample or standard.
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