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Catalog
C001586
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Alternative name
Leukemia inhibitory factor receptor CLIA KIT; CD_antigen: CD118LIFR CLIA KIT; LIF receptor CLIA KIT; LIF-R CLIA KIT
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GeneLIFR
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Other Species
Mouse LIFR clia kitRat LIFR clia kit
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Species ReactivityHuman
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SpecificityThis kit recognizes natural and recombinant LIFR. No significant cross-reactivity or interference between LIFR and analogues was observed.
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SamplesSerum, plasma and other biological fluids.
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Detection Range62.5-4000pg/mL
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Sensitivity37.5pg/mL
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Product Description specificalIntended Uses: This CLIA kit applies to the in vitro quantitative determination of LIFR concentrations in serum, plasma and other biological fluids.
Principle of the Assay||This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to LIFR. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for LIFR and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain LIFR, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of LIFR. You can calculate the concentration of LIFR in the samples by comparing the RLU value of the samples to the standard curve.
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