-
Catalog
C000152
-
GeneTRAIL
-
Other Species
Mouse TRAIL/TNFSF10 clia kitHuman TRAIL/TNFSF10 clia kitRat TRAIL/TNFSF10 clia kit
-
Species ReactivityHuman
-
SpecificityThis assay has high sensitivity and excellent specificity for detection of TRAIL. No significant cross-reactivity or interference between TRAIL and analogues was observed.
-
Detection Range41.2-30,000pg/mL. The standard curve concentrations used for the CLIA's were 30,000pg/mL, 10,000pg/mL, 3,333.3pg/mL, 1,111.1pg/mL, 370.4pg/mL, 123.5pg/mL, 41.2pg/mL.
-
SensitivityThe minimum detectable dose of TRAIL is typically less than 15.5pg/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean RLU value of 20 replicates of the zero standard added by their two standard deviations.
-
Product Description specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TRAIL. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to TRAIL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the TRAIL level in the sample or standard.
-
Related products with Human TRAIL clia kit